Smooth muscle cell recruitment to lymphatic vessels requires PDGFB and impacts vessel size but not identity.
Identifieur interne : 000175 ( Main/Exploration ); précédent : 000174; suivant : 000176Smooth muscle cell recruitment to lymphatic vessels requires PDGFB and impacts vessel size but not identity.
Auteurs : Yixin Wang [Suède] ; Yi Jin [Suède] ; Maarja Andaloussi M E [Suède] ; Yang Zhang [Suède] ; Henrik Orts Ter [Suède] ; Christer Betsholtz [Suède] ; Taija M Kinen [Suède] ; Lars Jakobsson [Suède]Source :
- Development (Cambridge, England) [ 1477-9129 ] ; 2017.
Abstract
Tissue-fluid drains through blind-ended lymphatic capillaries, via smooth muscle cell (SMC)-covered collecting vessels into venous circulation. Both defective SMC recruitment to collecting vessels and ectopic recruitment to lymphatic capillaries are thought to contribute to vessel failure, leading to lymphedema. However, mechanisms controlling lymphatic SMC recruitment and their role in vessel maturation are unknown. Here we demonstrate that platelet-derived growth factor B (PDGFB) regulates lymphatic SMC recruitment in multiple vascular beds. PDGFB is selectively expressed by lymphatic endothelial cells (LECs) of collecting vessels. LEC-specific deletion of Pdgfb prevented SMC recruitment causing dilation and failure of pulsatile contraction of collecting vessels. However, vessel remodelling and identity were unaffected. Unexpectedly, PDGFB overexpression in LECs did not induce SMC recruitment to capillaries. This was explained by the demonstrated requirement of PDGFB extracellular matrix (ECM) retention for lymphatic SMC recruitment, and low presence of PDGFB-binding ECM components around lymphatic capillaries. These results demonstrate a requirement of LEC-autonomous PDGFB expression and retention for SMC recruitment to lymphatic vessels and suggest an ECM-controlled checkpoint preventing SMC investment of capillaries, which is a common feature in lymphedematous skin.
DOI: 10.1242/dev.147967
PubMed: 28851707
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream PubMed, to step Corpus: 000030
- to stream PubMed, to step Curation: 000030
- to stream PubMed, to step Checkpoint: 000030
- to stream Ncbi, to step Merge: 009426
- to stream Ncbi, to step Curation: 009426
- to stream Ncbi, to step Checkpoint: 009426
- to stream Main, to step Merge: 000175
- to stream Main, to step Curation: 000175
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Smooth muscle cell recruitment to lymphatic vessels requires PDGFB and impacts vessel size but not identity.</title><author><name sortKey="Wang, Yixin" sort="Wang, Yixin" uniqKey="Wang Y" first="Yixin" last="Wang">Yixin Wang</name><affiliation wicri:level="1"><nlm:affiliation>Karolinska Institutet, Department of Medical Biochemistry and Biophysics, Division of Vascular Biology, Scheeles Väg 2, SE171 77 Stockholm, Sweden.</nlm:affiliation><country xml:lang="fr">Suède</country><wicri:regionArea>Karolinska Institutet, Department of Medical Biochemistry and Biophysics, Division of Vascular Biology, Scheeles Väg 2, SE171 77 Stockholm</wicri:regionArea><wicri:noRegion>SE171 77 Stockholm</wicri:noRegion></affiliation></author><author><name sortKey="Jin, Yi" sort="Jin, Yi" uniqKey="Jin Y" first="Yi" last="Jin">Yi Jin</name><affiliation wicri:level="1"><nlm:affiliation>Karolinska Institutet, Department of Medical Biochemistry and Biophysics, Division of Vascular Biology, Scheeles Väg 2, SE171 77 Stockholm, Sweden.</nlm:affiliation><country xml:lang="fr">Suède</country><wicri:regionArea>Karolinska Institutet, Department of Medical Biochemistry and Biophysics, Division of Vascular Biology, Scheeles Väg 2, SE171 77 Stockholm</wicri:regionArea><wicri:noRegion>SE171 77 Stockholm</wicri:noRegion></affiliation></author><author><name sortKey="M E, Maarja Andaloussi" sort="M E, Maarja Andaloussi" uniqKey="M E M" first="Maarja Andaloussi" last="M E">Maarja Andaloussi M E</name><affiliation wicri:level="4"><nlm:affiliation>Uppsala University, Dept. Immunology, Genetics and Pathology, Rudbeck Laboratory, Dag Hammarskjölds väg 20, SE751 85 Uppsala, Sweden.</nlm:affiliation><country xml:lang="fr">Suède</country><wicri:regionArea>Uppsala University, Dept. Immunology, Genetics and Pathology, Rudbeck Laboratory, Dag Hammarskjölds väg 20, SE751 85 Uppsala</wicri:regionArea><orgName type="university">Université d'Uppsala</orgName><placeName><settlement type="city">Uppsala</settlement><region nuts="1">Svealand</region><region nuts="1">East Middle Sweden</region></placeName></affiliation></author><author><name sortKey="Zhang, Yang" sort="Zhang, Yang" uniqKey="Zhang Y" first="Yang" last="Zhang">Yang Zhang</name><affiliation wicri:level="4"><nlm:affiliation>Uppsala University, Dept. Immunology, Genetics and Pathology, Rudbeck Laboratory, Dag Hammarskjölds väg 20, SE751 85 Uppsala, Sweden.</nlm:affiliation><country xml:lang="fr">Suède</country><wicri:regionArea>Uppsala University, Dept. Immunology, Genetics and Pathology, Rudbeck Laboratory, Dag Hammarskjölds väg 20, SE751 85 Uppsala</wicri:regionArea><orgName type="university">Université d'Uppsala</orgName><placeName><settlement type="city">Uppsala</settlement><region nuts="1">Svealand</region><region nuts="1">East Middle Sweden</region></placeName></affiliation></author><author><name sortKey="Orts Ter, Henrik" sort="Orts Ter, Henrik" uniqKey="Orts Ter H" first="Henrik" last="Orts Ter">Henrik Orts Ter</name><affiliation wicri:level="4"><nlm:affiliation>Uppsala University, Dept. Immunology, Genetics and Pathology, Rudbeck Laboratory, Dag Hammarskjölds väg 20, SE751 85 Uppsala, Sweden.</nlm:affiliation><country xml:lang="fr">Suède</country><wicri:regionArea>Uppsala University, Dept. Immunology, Genetics and Pathology, Rudbeck Laboratory, Dag Hammarskjölds väg 20, SE751 85 Uppsala</wicri:regionArea><orgName type="university">Université d'Uppsala</orgName><placeName><settlement type="city">Uppsala</settlement><region nuts="1">Svealand</region><region nuts="1">East Middle Sweden</region></placeName></affiliation></author><author><name sortKey="Betsholtz, Christer" sort="Betsholtz, Christer" uniqKey="Betsholtz C" first="Christer" last="Betsholtz">Christer Betsholtz</name><affiliation wicri:level="4"><nlm:affiliation>Uppsala University, Dept. Immunology, Genetics and Pathology, Rudbeck Laboratory, Dag Hammarskjölds väg 20, SE751 85 Uppsala, Sweden.</nlm:affiliation><country xml:lang="fr">Suède</country><wicri:regionArea>Uppsala University, Dept. Immunology, Genetics and Pathology, Rudbeck Laboratory, Dag Hammarskjölds väg 20, SE751 85 Uppsala</wicri:regionArea><orgName type="university">Université d'Uppsala</orgName><placeName><settlement type="city">Uppsala</settlement><region nuts="1">Svealand</region><region nuts="1">East Middle Sweden</region></placeName></affiliation></author><author><name sortKey="M Kinen, Taija" sort="M Kinen, Taija" uniqKey="M Kinen T" first="Taija" last="M Kinen">Taija M Kinen</name><affiliation wicri:level="4"><nlm:affiliation>Uppsala University, Dept. Immunology, Genetics and Pathology, Rudbeck Laboratory, Dag Hammarskjölds väg 20, SE751 85 Uppsala, Sweden Taija.Makinen@igp.uu.se Lars.Jakobsson@ki.se.</nlm:affiliation><country wicri:rule="url">Suède</country><wicri:regionArea>Uppsala University, Dept. Immunology, Genetics and Pathology, Rudbeck Laboratory, Dag Hammarskjölds väg 20, SE751 85 Uppsala</wicri:regionArea><orgName type="university">Université d'Uppsala</orgName><placeName><settlement type="city">Uppsala</settlement><region nuts="1">Svealand</region><region nuts="1">East Middle Sweden</region></placeName></affiliation></author><author><name sortKey="Jakobsson, Lars" sort="Jakobsson, Lars" uniqKey="Jakobsson L" first="Lars" last="Jakobsson">Lars Jakobsson</name><affiliation wicri:level="1"><nlm:affiliation>Karolinska Institutet, Department of Medical Biochemistry and Biophysics, Division of Vascular Biology, Scheeles Väg 2, SE171 77 Stockholm, Sweden Taija.Makinen@igp.uu.se Lars.Jakobsson@ki.se.</nlm:affiliation><country wicri:rule="url">Suède</country><wicri:regionArea>Karolinska Institutet, Department of Medical Biochemistry and Biophysics, Division of Vascular Biology, Scheeles Väg 2, SE171 77 Stockholm</wicri:regionArea><wicri:noRegion>SE171 77 Stockholm</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2017">2017</date><idno type="RBID">pubmed:28851707</idno><idno type="pmid">28851707</idno><idno type="doi">10.1242/dev.147967</idno><idno type="wicri:Area/PubMed/Corpus">000030</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000030</idno><idno type="wicri:Area/PubMed/Curation">000030</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">000030</idno><idno type="wicri:Area/PubMed/Checkpoint">000030</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">000030</idno><idno type="wicri:Area/Ncbi/Merge">009426</idno><idno type="wicri:Area/Ncbi/Curation">009426</idno><idno type="wicri:Area/Ncbi/Checkpoint">009426</idno><idno type="wicri:Area/Main/Merge">000175</idno><idno type="wicri:Area/Main/Curation">000175</idno><idno type="wicri:Area/Main/Exploration">000175</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Smooth muscle cell recruitment to lymphatic vessels requires PDGFB and impacts vessel size but not identity.</title><author><name sortKey="Wang, Yixin" sort="Wang, Yixin" uniqKey="Wang Y" first="Yixin" last="Wang">Yixin Wang</name><affiliation wicri:level="1"><nlm:affiliation>Karolinska Institutet, Department of Medical Biochemistry and Biophysics, Division of Vascular Biology, Scheeles Väg 2, SE171 77 Stockholm, Sweden.</nlm:affiliation><country xml:lang="fr">Suède</country><wicri:regionArea>Karolinska Institutet, Department of Medical Biochemistry and Biophysics, Division of Vascular Biology, Scheeles Väg 2, SE171 77 Stockholm</wicri:regionArea><wicri:noRegion>SE171 77 Stockholm</wicri:noRegion></affiliation></author><author><name sortKey="Jin, Yi" sort="Jin, Yi" uniqKey="Jin Y" first="Yi" last="Jin">Yi Jin</name><affiliation wicri:level="1"><nlm:affiliation>Karolinska Institutet, Department of Medical Biochemistry and Biophysics, Division of Vascular Biology, Scheeles Väg 2, SE171 77 Stockholm, Sweden.</nlm:affiliation><country xml:lang="fr">Suède</country><wicri:regionArea>Karolinska Institutet, Department of Medical Biochemistry and Biophysics, Division of Vascular Biology, Scheeles Väg 2, SE171 77 Stockholm</wicri:regionArea><wicri:noRegion>SE171 77 Stockholm</wicri:noRegion></affiliation></author><author><name sortKey="M E, Maarja Andaloussi" sort="M E, Maarja Andaloussi" uniqKey="M E M" first="Maarja Andaloussi" last="M E">Maarja Andaloussi M E</name><affiliation wicri:level="4"><nlm:affiliation>Uppsala University, Dept. Immunology, Genetics and Pathology, Rudbeck Laboratory, Dag Hammarskjölds väg 20, SE751 85 Uppsala, Sweden.</nlm:affiliation><country xml:lang="fr">Suède</country><wicri:regionArea>Uppsala University, Dept. Immunology, Genetics and Pathology, Rudbeck Laboratory, Dag Hammarskjölds väg 20, SE751 85 Uppsala</wicri:regionArea><orgName type="university">Université d'Uppsala</orgName><placeName><settlement type="city">Uppsala</settlement><region nuts="1">Svealand</region><region nuts="1">East Middle Sweden</region></placeName></affiliation></author><author><name sortKey="Zhang, Yang" sort="Zhang, Yang" uniqKey="Zhang Y" first="Yang" last="Zhang">Yang Zhang</name><affiliation wicri:level="4"><nlm:affiliation>Uppsala University, Dept. Immunology, Genetics and Pathology, Rudbeck Laboratory, Dag Hammarskjölds väg 20, SE751 85 Uppsala, Sweden.</nlm:affiliation><country xml:lang="fr">Suède</country><wicri:regionArea>Uppsala University, Dept. Immunology, Genetics and Pathology, Rudbeck Laboratory, Dag Hammarskjölds väg 20, SE751 85 Uppsala</wicri:regionArea><orgName type="university">Université d'Uppsala</orgName><placeName><settlement type="city">Uppsala</settlement><region nuts="1">Svealand</region><region nuts="1">East Middle Sweden</region></placeName></affiliation></author><author><name sortKey="Orts Ter, Henrik" sort="Orts Ter, Henrik" uniqKey="Orts Ter H" first="Henrik" last="Orts Ter">Henrik Orts Ter</name><affiliation wicri:level="4"><nlm:affiliation>Uppsala University, Dept. Immunology, Genetics and Pathology, Rudbeck Laboratory, Dag Hammarskjölds väg 20, SE751 85 Uppsala, Sweden.</nlm:affiliation><country xml:lang="fr">Suède</country><wicri:regionArea>Uppsala University, Dept. Immunology, Genetics and Pathology, Rudbeck Laboratory, Dag Hammarskjölds väg 20, SE751 85 Uppsala</wicri:regionArea><orgName type="university">Université d'Uppsala</orgName><placeName><settlement type="city">Uppsala</settlement><region nuts="1">Svealand</region><region nuts="1">East Middle Sweden</region></placeName></affiliation></author><author><name sortKey="Betsholtz, Christer" sort="Betsholtz, Christer" uniqKey="Betsholtz C" first="Christer" last="Betsholtz">Christer Betsholtz</name><affiliation wicri:level="4"><nlm:affiliation>Uppsala University, Dept. Immunology, Genetics and Pathology, Rudbeck Laboratory, Dag Hammarskjölds väg 20, SE751 85 Uppsala, Sweden.</nlm:affiliation><country xml:lang="fr">Suède</country><wicri:regionArea>Uppsala University, Dept. Immunology, Genetics and Pathology, Rudbeck Laboratory, Dag Hammarskjölds väg 20, SE751 85 Uppsala</wicri:regionArea><orgName type="university">Université d'Uppsala</orgName><placeName><settlement type="city">Uppsala</settlement><region nuts="1">Svealand</region><region nuts="1">East Middle Sweden</region></placeName></affiliation></author><author><name sortKey="M Kinen, Taija" sort="M Kinen, Taija" uniqKey="M Kinen T" first="Taija" last="M Kinen">Taija M Kinen</name><affiliation wicri:level="4"><nlm:affiliation>Uppsala University, Dept. Immunology, Genetics and Pathology, Rudbeck Laboratory, Dag Hammarskjölds väg 20, SE751 85 Uppsala, Sweden Taija.Makinen@igp.uu.se Lars.Jakobsson@ki.se.</nlm:affiliation><country wicri:rule="url">Suède</country><wicri:regionArea>Uppsala University, Dept. Immunology, Genetics and Pathology, Rudbeck Laboratory, Dag Hammarskjölds väg 20, SE751 85 Uppsala</wicri:regionArea><orgName type="university">Université d'Uppsala</orgName><placeName><settlement type="city">Uppsala</settlement><region nuts="1">Svealand</region><region nuts="1">East Middle Sweden</region></placeName></affiliation></author><author><name sortKey="Jakobsson, Lars" sort="Jakobsson, Lars" uniqKey="Jakobsson L" first="Lars" last="Jakobsson">Lars Jakobsson</name><affiliation wicri:level="1"><nlm:affiliation>Karolinska Institutet, Department of Medical Biochemistry and Biophysics, Division of Vascular Biology, Scheeles Väg 2, SE171 77 Stockholm, Sweden Taija.Makinen@igp.uu.se Lars.Jakobsson@ki.se.</nlm:affiliation><country wicri:rule="url">Suède</country><wicri:regionArea>Karolinska Institutet, Department of Medical Biochemistry and Biophysics, Division of Vascular Biology, Scheeles Väg 2, SE171 77 Stockholm</wicri:regionArea><wicri:noRegion>SE171 77 Stockholm</wicri:noRegion></affiliation></author></analytic><series><title level="j">Development (Cambridge, England)</title><idno type="eISSN">1477-9129</idno><imprint><date when="2017" type="published">2017</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Tissue-fluid drains through blind-ended lymphatic capillaries, via smooth muscle cell (SMC)-covered collecting vessels into venous circulation. Both defective SMC recruitment to collecting vessels and ectopic recruitment to lymphatic capillaries are thought to contribute to vessel failure, leading to lymphedema. However, mechanisms controlling lymphatic SMC recruitment and their role in vessel maturation are unknown. Here we demonstrate that platelet-derived growth factor B (PDGFB) regulates lymphatic SMC recruitment in multiple vascular beds. PDGFB is selectively expressed by lymphatic endothelial cells (LECs) of collecting vessels. LEC-specific deletion of Pdgfb prevented SMC recruitment causing dilation and failure of pulsatile contraction of collecting vessels. However, vessel remodelling and identity were unaffected. Unexpectedly, PDGFB overexpression in LECs did not induce SMC recruitment to capillaries. This was explained by the demonstrated requirement of PDGFB extracellular matrix (ECM) retention for lymphatic SMC recruitment, and low presence of PDGFB-binding ECM components around lymphatic capillaries. These results demonstrate a requirement of LEC-autonomous PDGFB expression and retention for SMC recruitment to lymphatic vessels and suggest an ECM-controlled checkpoint preventing SMC investment of capillaries, which is a common feature in lymphedematous skin.</div></front></TEI><affiliations><list><country><li>Suède</li></country><region><li>East Middle Sweden</li><li>Svealand</li></region><settlement><li>Uppsala</li></settlement><orgName><li>Université d'Uppsala</li></orgName></list><tree><country name="Suède"><noRegion><name sortKey="Wang, Yixin" sort="Wang, Yixin" uniqKey="Wang Y" first="Yixin" last="Wang">Yixin Wang</name></noRegion><name sortKey="Betsholtz, Christer" sort="Betsholtz, Christer" uniqKey="Betsholtz C" first="Christer" last="Betsholtz">Christer Betsholtz</name><name sortKey="Jakobsson, Lars" sort="Jakobsson, Lars" uniqKey="Jakobsson L" first="Lars" last="Jakobsson">Lars Jakobsson</name><name sortKey="Jin, Yi" sort="Jin, Yi" uniqKey="Jin Y" first="Yi" last="Jin">Yi Jin</name><name sortKey="M E, Maarja Andaloussi" sort="M E, Maarja Andaloussi" uniqKey="M E M" first="Maarja Andaloussi" last="M E">Maarja Andaloussi M E</name><name sortKey="M Kinen, Taija" sort="M Kinen, Taija" uniqKey="M Kinen T" first="Taija" last="M Kinen">Taija M Kinen</name><name sortKey="Orts Ter, Henrik" sort="Orts Ter, Henrik" uniqKey="Orts Ter H" first="Henrik" last="Orts Ter">Henrik Orts Ter</name><name sortKey="Zhang, Yang" sort="Zhang, Yang" uniqKey="Zhang Y" first="Yang" last="Zhang">Yang Zhang</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Sante/explor/LymphedemaV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000175 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 000175 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Sante
|area= LymphedemaV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:28851707
|texte= Smooth muscle cell recruitment to lymphatic vessels requires PDGFB and impacts vessel size but not identity.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:28851707" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a LymphedemaV1
| This area was generated with Dilib version V0.6.31. |  |